Dear Subscriber,
Host cell proteins (HCP) are a major class of impurities derived from recombinant protein production processes. Their presence in therapeutic antibody-based drugs can reduce drug efficacy and induce adverse reactions in patients. HCP levels are typically measured by enzyme-linked immunosorbent assays (ELISA). However, mass spectrometry-based accurate quantification and fine characterization of HCP are emerging as complementary methods.
In this context, Dr. Christine Carapito and her team at the Bio-Organic Mass Spectrometry Laboratory (LSMBO) at the University of Strasbourg, have developed LC-MS/MS methods relying on data-independent acquisition (DIA) and have benchmarked these methods against pure targeted approaches coupled with isotope dilution for absolute quantification. Biognosys' Spectronaut software was a key asset to her and her team to detect and quantify trace-level proteins as HCP.
