Dear Subscriber,
At Biognosys, we are committed to the continuous development of innovative products for accurate quantitative proteomics that are easily implemented in your facility. Our software packages Spectronaut™, SpectroDive™, and SpectroMine™ support a large variety of proteomics workflows including data-independent acquisition (DIA), targeted proteomics (SureQuant/PRM/SRM), and isobaric label experiments for quantitative analysis. Below we highlight recent examples demonstrating how our software solutions enabled researchers to complete cutting-edge projects in systems biology. Deep Phosphoproteome Profiling with directDIA, Spectronaut’s Library-free Workflow
Bekker-Jensen D.B. et al. Nature Communications 2020
Protein phosphorylation is a predominant post-translational modification that dynamically regulates protein function and cell signaling. In this collaboration with the Olsen group at the University of Copenhagen, we showed that DIA allows reliable phosphosite localization for hundreds of samples and outperforms DDA in dynamic range, reproducibility, sensitivity, and accuracy. Additionally, directDIA, the library-free workflow of Spectronaut reaches phosphoproteome depths comparable to library-based DIA analysis.
Read the complete publication here.
Identification of Drug Targets in Complex Proteomes with Spectronaut and SpectroMine
Piazza I. et al. Nature Communications 2020
Chemoproteomics enables protein target identification of bioactive compounds, unraveling the mode of action of drugs. In this joint effort with Prof. Picotti’s group, ETH Zurich, we presented a novel chemoproteomic workflow combining LiP and machine learning-based data analysis. This next-generation proteomics approach enables the identification of small molecule drug targets in complex proteomes and the analysis of their binding properties across species and drug target classes.
Read the complete publication here.
Analysis of Oxytocin Response in Autism with SpectroMine
Hörnberg H. et al. Nature 2020
The oxytocin system is evolutionarily conserved, has important functions in the control of social behaviors, and has been implicated in the context of autism spectrum disorders (ASD). In this work, Dr. Hörnberg and colleagues used tandem mass tags (TMT) proteomics to unravel molecular alterations of the oxytocin signaling in dopaminergic neurons, and suggest that pharmacological interventions targeting such core processes may restore molecular signaling and benefit a wide range of patients with ASD.
Read the complete publication here.
Phosphoproteomics Survey of SARS-CoV-2 Infection with Spectronaut
Bouhaddou M. et al. Cell 2020
This publication is an important joint effort of the scientific community to provide a quantitative mass spectrometry-based phosphoproteomics survey of SARS-CoV-2, the causative agent of coronavirus disease 2019 (COVID-19). The study showed that SARS-CoV-2 infection causes a dramatic rewiring of host and viral protein phosphorylation, stimulates filopodial protrusions, and induces cell cycle arrest. Finally, the authors identified potential antiviral drugs using a panel of kinase inhibitors.
Read the complete publication here.
